Beilstein J. Nanotechnol.2014,5, 2016–2025, doi:10.3762/bjnano.5.210
for this purpose is intravital 2-photonmicroscopy (2PM) based on tissue autofluorescence [1]. Volumes of up to 600 × 600 × 150 µm3 can be scanned within less than 30 seconds, and repeated scans allow for individual particles to be tracked over minutes or even hours. However, digital images recorded
characteristics improves the preservation of fine structures such as nanoparticles while less artefacts were produced as compared to reference algorithms. Our method is applicable to other imaging modalities as well, provided the specific noise characteristics are known and taken into account.
Keywords: 2-photon
microscopy (2PM); denoising; in vivo imaging; nanoparticles; signal to noise ratio (SNR); quantum dots; Introduction
Imaging methods applied to detect fluorescent nanoparticles in mucosal tissues should provide high optical resolution and allow large volumes to be scanned. An important and versatile tool
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Figure 1:
Enlarged optical section of gut tissue in intravital 2-photon microscopy: a) ground-truth signal, b...